The columns ended up washed sequentially with buffer one and buffer 1 that contains twenty five mM imidazole. Bound protein was eluted with buffer one that contains 500 mM imidazole and loaded on to 16/60 HiLoad Superdex-75 columns (GE Healthcare)

A composition dependent alignment reveals significant functions in the Start off domain subfamilies, and highlights critical conserved residues involved in ligand binding. Of unique curiosity in the scope of this…