Antibodyantigen complexes were detected by chemiluminescence using ECLTM Select Western Blotting Detection Package (GE Health care) as indicated in manufacturer’s guidelines
In vitro differentiated adipocytes had been treated with 40 nM of miRIDIAN miRNA Mimics (Thermo Fisher Scientific, Lafayette, CO) and HiperFect Transfection Reagent (Qiagen, Hilden, Germany) at day ten?2 of…