Ich is attributed to miR-206-mediated HDAC6 inhibition. Our analysis suggests that the use of USCs-Exo represents a novel therapeutic technique for stroke recovery. Funding: This perform was funded by National Natural Science Foundation of China (Numbers 81671209, 81471243 and 81472152).PF11.Protective impact of extracellular vesicles released from the neural stem cells on 6-hydroxydopamine induced pathological condition of Parkinson’s illness Eun Ji Leea, Dowon Hwangb and Dong Soo Leea Division of Nuclear Medicine, Seoul National University Hospital, Seoul, Republic of Korea; bSeoul National University Hospital, Seoul, Republic of Koreaapotential therapeutic sources for PD, NSCsecreted extracellular vesicles (EVs) such as exosomes are essential mediators of optimistic paracrine effects. Direct evidence for neuronal protective effects of EVs is crucial for developing new PD therapeutics. Methods: To trace EV movement, a lentivirus containing Palm-tandem dimer tdTomato (Palm-td) was transduced into F3 NSCs. EVs isolated from Palm-tdinfected F3 cells showed high tdTomato fluorescence intensity enough to visualize their functional actions including secretion, migration and engulfment amongst cells. We found that pretreatment with EVs dramatically prevented 6-OHDA-induced toxicity by decreasing intracellular reactive oxygen species (ROS), percentage of apoptotic cells and caspase-3/7 activity. Outcomes: These outcomes indicate that NSC-derived EVs have neuroprotective effects against the cell harm, possibly through antioxidant and anti-apoptotic action. Particularly, Glycophorin-A/CD235a Proteins supplier F3-derived EVs correctly prevents 6OHDA-induced the production of ROS, NSC-EVs that inhibit ROS production may well be applied as an important therapeutic agent for neuroprotection. EVmediated neuroprotection likely acts by inhibiting the generation of caspase-3/7, top to lower apoptosis induction triggered by 6-OHDA neurotoxicity. Summary/Conclusion: In summary, this study demonstrates that NSC-derived EVs protected dopaminergic cells from oxidative insults. When 6-OHDA-induced cell death in SH-SY5Y cells happens by the generation of ROS, the neurotoxin-mediated cell death was suppressed by F3-derived EVs through their protective effects on ROS-induced cell damage. We for that reason expect that further investigations into the therapeutic applications of NSC-derived EVs will reveal added positive aspects for EV-based PD therapies in comparison to cell transplantation.PF11.The function of extracellular vesicles secreted by human-induced Retinoic Acid Receptor-Related Orphan Receptors Proteins Storage & Stability pluripotent stem cell-derived mesenchymal stem cells on a cellular ischemic stroke model Gang Lu, Man Sze Wong, Xian Wei Su, Rui Can Cao, Hoi Hung Cheung and Wai Yee Chan CUHK-SDU joint laboratory on reproductive genetics, College of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, Hong KongIntroduction: Parkinson’s illness (PD) is really a neurodegenerative disease characterized by bradykinesia, resting tremors and postural instability. A important symptom of PD is the loss with the nigral dopaminergic neurons and subsequent dopamine deficit inside the brain. However, the precise mechanism continues to be unknown. When neural stem cells (NSCs) areIntroduction: Stroke is the second leading reason for death plus the principal cause of long-term disability, but but lack of efficient treatment. Research suggested the transplantation of mesenchymal stem cells (MSCs) enhanced recovery from stroke in animalJOURNAL OF EXTRACELLULAR VESICLESmodels, equivalent therapeutic impact was fou.