O substantial differences amongst Scaffold Library medchemexpress meadows and time with regards to the abortedPlants 2021, 10,7 of3.three. Spatio-Temporal Variability in the Flowering Work and Reproductive Phenology Flowering effort didn’t significantly differ amongst meadows and time (Table three; Figure 5A), displaying an average of 781 157 spathes m-2 per Z. noltei meadow. Similarly, there have been no significant variations among meadows and time regarding the aborted spathes and the percentages of spathes in stages I, III and V (Table 3; Figure 5B,D,F,G). Plants 2021, ten, x FOR PEER Critique 15 over However, the percentage of spathes in stages II and IV were substantially variableof 15 time (Table three; Figure 5C,E).Figure 5. Flowering effort (A), percentages of spathes in every single reproductive stage (B ) and percentFigure five. Flowering effort (A), percentages of spathes in every reproductive stage (B ) and percentage age of aborted spathes (G) time (mean SE, n n = of aborted spathes (G) overover time (imply SE,= four).four).three.four. Germination Capacity A total of 158 seeds have been obtained in the cultured spathes. Seed production did not differ substantially amongst meadows (M1 two, t = 0.443, p = 0.669; M1 three, t = 0.482, p = 0.643 and M2 three, t = 0.786, p = 0.455), averaging 0.422 0.109 seeds per collected spathe. Amid these seeds, 51.26 (81 seeds) have been fully matured and applied for the germination testPlants 2021, 10,8 ofTable three. Final results obtained in the two-way ANOVA analyses for FE and stages of sexual reproduction. Benefits are expressed as F-values and connected p-values (in parenthesis). Significant results are in bold. The significance level in all tests was = 0.01. Variable Flowering work Stage I Stage II Stage III Stage IV Stage V Aborted spathes Meadow (df = three) two.853 (0.047) 1.274 (0.294) 0.408 (0.748) 0.287 (0.834) 1.150 (0.339) 1.266 (0.297) 0.774 (0.514) Time (df = three) 2.495 (0.071) 2.290 (0.090) ten.37 (0.001) 1.470 (0.235) 10.92 (0.001) 1.595 (0.203) 3.375 (0.026) Meadow Time (df = 9) 0.708 (0.699) 1.782 (0.097) 1.010 (0.446) 2.282 (0.032) 0.807 (0.612) 1.047 (0.418) 0.534 (0.842)three.four. Germination Ability A total of 158 seeds have been obtained in the cultured spathes. Seed production didn’t differ significantly among meadows (M1 2, t = 0.443, p = 0.669; M1 3, t = 0.482, p = 0.643 and M2 3, t = 0.786, p = 0.455), averaging 0.422 0.109 seeds per collected spathe. Amid these seeds, 51.26 (81 seeds) had been totally matured and utilised for the germination test (Table S2). Similarly, germination capacity didn’t show important differences among meadows (Table four), germinating 33.73 of mature seeds. The average germination time with the seeds formed in M1, M2 and M3 was 1.000 0.001, 1.389 0.164 and 2.200 0.490, respectively.Table four. Percentage of germinated and no germinated seeds. Comparison among meadows was carried out by means of Fisher’s precise test of independence. Meadow M1 M2 M3 Fisher’s precise test of independence Germinated 24 43.9 33.3 2.69 No Germinated 76 56.1 66.7 p = 0.4. Discussion This study shows the first data on reproductive phenology of Zostera noltei inside the Ria de Aveiro lagoon. The flowering period of Z. noltei in Ria de Aveiro began about June and lasted until near November, Benidipine Formula peaking amongst July and August. Flowering effort and reproductive phenology were similar among meadows and over time, suggesting that all meadows evolved similarly over the flowering period. The proportion of newly formed spathes (Stage I), apparently fertilised spathes (Stage III) and spathes with mature seeds (Stage V) was co.