Name: E.coli Lactose Operon Repressor/LacI Recombinant Protein (His Tag)
Synonyms: Lactose operon repressor;LacI
Expression host: E.coli
Sequence: Met1-Gln360
Accesstion: P03023
Species: E.coli
Mol_Mass: 39.4 kDa
AP_Mol_Mass: 40 kDa
Tag: C-His
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin:
Storage: Store at
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.
Reconstitution: Not Applicable
Background: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
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