Name: Human DNA Polymerase β/POLB Recombinant Protein (His Tag)

Synonyms: DNA Polymerase Beta;POLB

Expression host: E.coli

Sequence: Ser2-Glu335

Accesstion: P06746

Species: Human

Mol_Mass: 39.2 kDa

AP_Mol_Mass: 39 kDa

Tag: C-His

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin:

Storage: Store at

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8.

Reconstitution: Not Applicable

Background: Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5′-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5′ sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3′ end of the arising single-nucleotide gap. It conducts ‘gap-filling’ DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.

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