Name: Thermobifida fusca Cutinase Recombinant Protein (His Tag)
Synonyms: Cutinase
Expression host: E.coli
Sequence: Ala1-Phe261
Accesstion: E5BBQ3
Species: Thermobifida fusca
Mol_Mass: 29.5 kDa
AP_Mol_Mass: 28-30 kDa
Tag: C-His
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin:
Storage: Store at
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at
Formulation: Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 50% Glycerol, 5% Mannitol, 0.02% Tween 80, pH4.5.
Reconstitution: Not Applicable
Background: Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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