Name: Streptomyces hygroscopicus Bar Recombinant Protein
Synonyms: Phosphinothricin N-acetyltransferase;PPT N-acetyltransferase;Phosphinothricin-resistance protein;bar
Expression host: E.coli
Sequence: Met1-Ile183
Accesstion: P16426
Species: Streptomyces hygroscopicus
Mol_Mass: 20.6 kDa
AP_Mol_Mass: 18-20 kDa
Tag: None
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin:
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Formulation: Lyophilized from a 0.2 μm filtered solution of 12.5mM Tris-HCl, 50mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween 80, 2mM DTT, 1mM EDTA, pH8.5.Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
GOT1 ProteinSynonyms
CEACAM1 Proteinmanufacturer
Popular categories:
CCL16
Hemagglutinin-Neuraminidase