Name: Human ATG10 Recombinant Protein (His&T7 Tag)
Synonyms: Ubiquitin-Like-Conjugating Enzyme ATG10;Autophagy-Related Protein 10;APG10-Like;ATG10
Expression host: E.coli
Sequence: Met 1-Thr190
Accesstion: Q9H0Y0
Species: Human
Mol_Mass: 24.5 kDa
AP_Mol_Mass: 30 kDa
Tag: N-T7 & C-His
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin:
Storage: Store at
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 20% Glycerol, 0.02% Tween 80, 3mM TCEP, pH 8.5.
Reconstitution: Not Applicable
Background: Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7.
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