Name: Human LCAT Recombinant Protein (His Tag)
Synonyms: Phosphatidylcholine-sterol acyltransferase;also named Lecithin-cholesterol acyltransferase;Phospholipid-cholesterol acyltransferase and LACT;is an extracellular cholesterol esterifying enzyme which belongs to the AB hydrolase superfamily.
Expression host: HEK293 Cells
Sequence: Phe25-Glu440
Accesstion: P04180
Species: Human
Mol_Mass: 48.1 kDa
AP_Mol_Mass: 66 kDa
Tag: C-His
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin:
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Formulation: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Lipase family. The gene encoding this protein is expressed mainly in brain, liver and testes,followed by secreting into plasma and cerebral spinal fluid. The esterification of cholesterol is required for cholesterol transport. LCAT is a central enzyme in the extracellular metabolism of plasma lipoproteins. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD) and a cause of fish-eye disease (FED).
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