Way had been identified by KEGG enrichment annotation (Fig. four: five). KEGG evaluation showed
Way had been identified by KEGG enrichment annotation (Fig. four: five). KEGG analysis showed that compared with CAK (BR spraying for 0 h), the expression of your UTPglucose-1-phosphate uridylyltransferase (UGP), SPS, glucose-6-phosphate isomerase (GPI), pyrophosphateJin et al. BMC Genomics(2022) 23:Page 10 ofFig. 5 A feasible model on the BR Wnt Storage & Stability signaling pathway with BRs (the activation state of BR signaling) sprayed onto tea leavesJin et al. BMC Genomics(2022) 23:Web page 11 offructose-6-phosphate 1-phosphotransferase (PFP), and epidermis-specific secreted glycoprotein (EP) key regulatory genes related to the sucrose biosynthesis pathway have been upregulated right after BR spraying for three h, 9 h, 24 h, and 48 h.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes in the biosynthetic pathway of flavonoidsEleven genes involved in flavonoid biosynthesis have been identified by KEGG enrichment annotation (Fig. four: 6). The flavonoid biosynthesis-related genes PAL, C4H, 4CL, chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid three,5-hydroxylase (F3’5’H), DFR, LAR, ANR, and UFGT were upregulated, with peak values observed at 48 h.DiscussionBR signal CDC site transduction mechanism in tea leavesThrough KEGG enrichment and annotation, 26 genes involved inside the BR signal transduction pathway have been identified. As outlined by the heat maps of genes connected to BR signal transduction beneath diverse BR remedies, it was identified that 26 genes in the BR signal transduction pathway have been considerably upregulated with escalating BR spraying time. Combined together with the BR signal transduction maps of Arabidopsis and rice, we describe a probable model with the BR signal pathway in tea leaves [291] (Fig. 5). At present, the signal transduction pathway of BR in Arabidopsis and rice has been reported. Compared with rice, the signal transduction pathway of BR in tea leaves is equivalent to that of Arabidopsis [24]. In contrast to the BR signal transduction pathway in a. thaliana, BAK1-like kinase includes each SERK and TMK4 in the BR signal transduction pathway of tea leaves. In our transcriptome information, the ATBS1-interacting components (AIF) and paclobutrazol resistance 1 (PRE) genes did not significantly differ in expression levels, whereas that of your teosinte branched (TCP) gene was important. AIF would be the unfavorable regulator of BR signal transduction, though PRE and TCP would be the optimistic regulators of BR signal transduction [34]. The outcomes showed that TCP, the forward regulator of BR signal transduction, plays a top part within the effects on the exogenous spraying of BRs onto young tea leaves.Exogenous spraying of BR promotes the growth and improvement of tea plantsGBSS, and SBE genes connected to starch synthesis; along with the flavonoid biosynthesis-related PAL, C4H, 4CL, CHS, CHI, F3H, F3’5, DFR, LAR, ANR, and UFGT genes were identified. The results showed that exogenous spraying of BRs upregulated the expression of genes associated to sucrose synthesis, chlorophyll synthesis, starch synthesis, and flavonoid biosynthesis. It might be inferred that exogenous BR spraying elevated the content of sucrose, chlorophyll, starch, and flavonoids. Furthermore, a sizable quantity of hugely expressed cyclin genes, such as Cyc, CycD3, CycD4, and CDC6, had been identified. Cell cycle regulatory proteins can bind to cell differentiation cycle-coding proteins and activate corresponding protein kinases, as a result advertising cell division. BRs can enhance plant development by advertising cell division.