GO:0019438) biosynthesis processes. Although the differentially expressed genes encoded for a number of amino acids were reported including glycine, alanine, glutamate, and aspartate, the aromatic and branched chain family amino acids were most affected. The branched chain amino acids were valine, leucine, and Brefeldin A biological activity isoleucine while aromatic amino acids included phenylalanine, tyrosine, and tryptophan. Tryptophan represented the most affected amino acids among the aromatic group as the expression of high number of genes associated with tryptophan precursor anthranilate biosynthesis and metabolisms were altered. Moreover, the specific downregulation of tryptophan biosynthesis (GO:0000162) and tryptophan CEP-37440 web metabolic process (GO:6568) were due to PEN as seen in both PEN- and DM3PEN-treated groups. For alanine biosynthesis, one unique gene (SP_1671, D-alanyl-alanine synthetase A) was downregulated in both DM3 and DM3PEN-treated PRSP but not in PEN-treated group (Tables S1 3). PEN-treated cells observed greater pathway differences as seen with the doubling of the number of enriched pathways found as compared to the DM3-treated cells (Tables S1 and S2). Several of these were associated with indolalklyamine, indole, and indole derivatives-related pathways, heterocycle biosynthesis, chorismate metabolic process, lyase, dicarboxylic acid metabolic and biosynthetic processes. Similar results were observed in DM3PENScientific RepoRts | 6:26828 | DOI: 10.1038/srepwww.nature.com/scientificreports/Figure 1. Heatmaps showing expression level of clustered genes of PRSP. Each group is classified into five clusters. (A) untreated versus DM3, (B) untreated versus PEN, and (C) untreated versus DM3PEN. and this was likely be due to presence of PEN in the combination treatment which produced such effects in the cells. For PSSP, the set of differentially expressed genes in all three groups were similar, observing predominant effect against the 30S small ribosomal subunit involving significant upregulation of the genes rrnaB16S, rrnaC16S, rrnaC23S, and rrnaD23S. Upregulation of rrnaC16S and 23S rrnaD23S rRNA genes were particularly drastic with more than 32-fold change as compared to the untreated cells except the lower upregulation fold-change in rrnaB16S of DM3PEN group.Effects of DM3 and combination treatment on nucleic acid metabolism. Results showed significant differential expression associated with genes related to DNA replication and transcription mechanisms. Notably, genes encoded for DNA helicase, gyrase, and topoisomerases subunits were differentially expressed. Different subunits of the DNA-directed RNA polymerase were found to be differentially expressed withScientific RepoRts | 6:26828 | DOI: 10.1038/srepwww.nature.com/scientificreports/Figure 2. Heatmaps showing expression level of clustered genes of PSSP. Each group is classified into five clusters. (A) untreated versus DM3, (B) untreated versus PEN, and (C) untreated versus DM3PEN. PEN-treatment; while both alpha- and beta-subunits were upregulated, the delta-subunit was downregulated. This is accompanied by upregulation of RNA polymerase sigma factor RpoD. Conversely, RpoD was downregulated in DM3-treated cells and no differential expression was observed with DNA-binding RNA polymerase subunits indicating that DM3 has no inhibitory activity on RNA polymerase. In the combination treatment, the collective effects were noted with upregulation of DNA-directed RNA-polymerase beta subunit while both alphaa.GO:0019438) biosynthesis processes. Although the differentially expressed genes encoded for a number of amino acids were reported including glycine, alanine, glutamate, and aspartate, the aromatic and branched chain family amino acids were most affected. The branched chain amino acids were valine, leucine, and isoleucine while aromatic amino acids included phenylalanine, tyrosine, and tryptophan. Tryptophan represented the most affected amino acids among the aromatic group as the expression of high number of genes associated with tryptophan precursor anthranilate biosynthesis and metabolisms were altered. Moreover, the specific downregulation of tryptophan biosynthesis (GO:0000162) and tryptophan metabolic process (GO:6568) were due to PEN as seen in both PEN- and DM3PEN-treated groups. For alanine biosynthesis, one unique gene (SP_1671, D-alanyl-alanine synthetase A) was downregulated in both DM3 and DM3PEN-treated PRSP but not in PEN-treated group (Tables S1 3). PEN-treated cells observed greater pathway differences as seen with the doubling of the number of enriched pathways found as compared to the DM3-treated cells (Tables S1 and S2). Several of these were associated with indolalklyamine, indole, and indole derivatives-related pathways, heterocycle biosynthesis, chorismate metabolic process, lyase, dicarboxylic acid metabolic and biosynthetic processes. Similar results were observed in DM3PENScientific RepoRts | 6:26828 | DOI: 10.1038/srepwww.nature.com/scientificreports/Figure 1. Heatmaps showing expression level of clustered genes of PRSP. Each group is classified into five clusters. (A) untreated versus DM3, (B) untreated versus PEN, and (C) untreated versus DM3PEN. and this was likely be due to presence of PEN in the combination treatment which produced such effects in the cells. For PSSP, the set of differentially expressed genes in all three groups were similar, observing predominant effect against the 30S small ribosomal subunit involving significant upregulation of the genes rrnaB16S, rrnaC16S, rrnaC23S, and rrnaD23S. Upregulation of rrnaC16S and 23S rrnaD23S rRNA genes were particularly drastic with more than 32-fold change as compared to the untreated cells except the lower upregulation fold-change in rrnaB16S of DM3PEN group.Effects of DM3 and combination treatment on nucleic acid metabolism. Results showed significant differential expression associated with genes related to DNA replication and transcription mechanisms. Notably, genes encoded for DNA helicase, gyrase, and topoisomerases subunits were differentially expressed. Different subunits of the DNA-directed RNA polymerase were found to be differentially expressed withScientific RepoRts | 6:26828 | DOI: 10.1038/srepwww.nature.com/scientificreports/Figure 2. Heatmaps showing expression level of clustered genes of PSSP. Each group is classified into five clusters. (A) untreated versus DM3, (B) untreated versus PEN, and (C) untreated versus DM3PEN. PEN-treatment; while both alpha- and beta-subunits were upregulated, the delta-subunit was downregulated. This is accompanied by upregulation of RNA polymerase sigma factor RpoD. Conversely, RpoD was downregulated in DM3-treated cells and no differential expression was observed with DNA-binding RNA polymerase subunits indicating that DM3 has no inhibitory activity on RNA polymerase. In the combination treatment, the collective effects were noted with upregulation of DNA-directed RNA-polymerase beta subunit while both alphaa.