Un several occasions with parameters on the eutionary model getting selected from unique prior distributions. In an effort to determine which model supplied the very best fitting priors, models were tested applying 
Bayes Variables. A hyperprior approach with mean-values of your exponential priors derived from a uniform distribution amongst to was determined to match best the information. The outcomes on the evaluation were visualized in Tracer vColors define groups: yellow are single-celled cyanobacteria of section I; orange single-celled from section II; green are multicellular, undifferentiated cyanobacteria from section III; blue are multicellular and differentiated bacteria from section IV; PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27364926?dopt=Abstract and pink from section V. Sections as described by CastenholzAC, B, C, E and E denote clades discussed in the text. Further file : Results from the test of substitutional saturation. Substitutional saturation of the 2,3,4,Amezinium metilsulfate site 5-Tetrahydroxystilbene 2-O-D-glucoside sequences was tested making use of DAMBE application. The index of substitutional saturation is smaller than the estimated important worth irrespective on the symmetry of the tree. The sequences are hence not saturated. Extra file : Ancestral character state reconstruction using maximum parsimony. Summary of outcomes over , randomly sampled trees from the Bayesian evaluation. Uniquely most effective states have been counted and are shown on the Bayesian consensus tree. Feasible states are unicellular (yellow) and multicellular (black). In the nodes, probabilities for every single character state are represented having a pie chart. The white part within the pie charts indicates fraction of trees where the node was absent, grey parts describe fraction of trees exactly where each states had been equally likely. Nodes where transitions occurred were labelled with an asterisk if they show strong support in the phylogenetic analyses. The maximum parsimony evaluation developed a comparable outcome in 
comparison with the maximum likelihood evaluation. A unicellular ancestry for the most recent popular ancestor of all cyanobacteria is supported. Nodes , and are most often optimized as multicellular. Multicellularity has been estimated for nodes and in trees and for node in trees. In contrast, single celled states for these nodes have already been reported, for node in out of , trees and for node and in out of , trees. 5 reversals to unicellularity may be detected and at least one particular reversal to multicellularity. Extra file : Phylogenetic tree of cyanobacteria – newick format. Phylogenetic tree of , cyanobacterial sequences including six chloroplasts and six Eubacteria analyzed applying maximum likelihood analysis having a GTR+G+I estimated substitution model, conducted with the application RAxML. Added file : Taxon names of the phylogenetic tree of cyanobacteria. Species names utilised inside the phylogenetic analysis carried out with RAxML software. Taxon names are ordered by subgroups as in Figure .Added materialAdditional file : Rooted Bayesian consensus tree of eubacterial species like five cyanobacterial species. Bayesian analysis of S rRNA gene sequences from Eubacteria, based on GTR+I+G substitution model with an archaean outgroup. Posterior probabilities (black) and bootstrap values (red) from re-samplings are displayed in the nodes. Cyanobacteria (blue-green box) are strongly supported as a monophyletic group with Gloeobacter violaceus being closest to other eubacterial species. Extra file : Bayesian consensus trees of cyanobacterial subset and unique outgroups – newick format. Bayesian consensus trees with posterior p.Un numerous occasions with parameters from the eutionary model getting chosen from distinctive prior distributions. In an effort to figure out which model provided the ideal fitting priors, models were tested utilizing Bayes Variables. A hyperprior strategy with mean-values from the exponential priors derived from a uniform distribution among to was determined to fit most effective the data. The outcomes of your analysis were visualized in Tracer vColors define groups: yellow are single-celled cyanobacteria of section I; orange single-celled from section II; green are multicellular, undifferentiated cyanobacteria from section III; blue are multicellular and differentiated bacteria from section IV; PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27364926?dopt=Abstract and pink from section V. Sections as described by CastenholzAC, B, C, E and E denote clades discussed within the text. Additional file : Results in the test of substitutional saturation. Substitutional saturation from the sequences was tested making use of DAMBE software program. The index of substitutional saturation is smaller sized than the estimated vital value irrespective in the symmetry of your tree. The sequences are thus not saturated. Added file : Ancestral character state reconstruction applying maximum parsimony. Summary of results more than , randomly sampled trees in the Bayesian evaluation. Uniquely most effective states were counted and are shown on the Bayesian consensus tree. Doable states are unicellular (yellow) and multicellular (black). In the nodes, probabilities for every character state are represented using a pie chart. The white aspect in the pie charts indicates fraction of trees exactly where the node was absent, grey components describe fraction of trees exactly where each states had been equally probably. Nodes where transitions occurred had been labelled with an asterisk if they show powerful support from the phylogenetic analyses. The maximum parsimony analysis made a equivalent outcome in comparison with the maximum likelihood analysis. A unicellular ancestry for probably the most recent popular ancestor of all cyanobacteria is supported. Nodes , and are most often optimized as multicellular. Multicellularity has been estimated for nodes and in trees and for node in trees. In contrast, single celled states for these nodes happen to be reported, for node in out of , trees and for node and in out of , trees. 5 reversals to unicellularity might be detected and at the least one particular reversal to multicellularity. More file : Phylogenetic tree of cyanobacteria – newick format. Phylogenetic tree of , cyanobacterial sequences such as six chloroplasts and six Eubacteria analyzed working with maximum likelihood analysis with a GTR+G+I estimated substitution model, carried out together with the software RAxML. Added file : Taxon names of the phylogenetic tree of cyanobacteria. Species names applied in the phylogenetic analysis carried out with RAxML computer software. Taxon names are ordered by subgroups as in Figure .Added materialAdditional file : Rooted Bayesian consensus tree of eubacterial species which includes 5 cyanobacterial species. Bayesian evaluation of S rRNA gene sequences from Eubacteria, based on GTR+I+G substitution model with an archaean outgroup. Posterior probabilities (black) and bootstrap values (red) from re-samplings are displayed in the nodes. Cyanobacteria (blue-green box) are strongly supported as a monophyletic group with Gloeobacter violaceus getting closest to other eubacterial species. Additional file : Bayesian consensus trees of cyanobacterial subset and distinctive outgroups – newick format. Bayesian consensus trees with posterior p.