For hypoxia therapies, cells have been positioned in an enclosed bactron anaerobic chamber (Shel Lab) that contains a 37uC temperature controlled incubator
Primers ended up annealed to a wild variety HA-EphA2 plasmid, and PCR was done with PfuUltra HF DNA polymerase (Stratagene). Nonmutated parental DNA was cleaved with DpnI restriction enzyme and…