Free thyroid hormone concentrations have been assayed by schedule ELISA procedures, as very well as TSH (third technology) and Thyroid Peroxidase and Thyroglobulin autoantibodies
In order to quantify the collagen recovered, a additional incubation (3 h at 4uC) with the human recombinant LAIR-two-Fc build (twenty microg/ml) was carried out, adopted by washing and addition…